This technical guidance factsheet was developed as part of a series of practical on-farm hatchery management guides based on the FAO Artemia Manual. It provides a concise, user-friendly reference for hatchery operators on the disinfection of Artemia cysts prior to incubation. The factsheet presents a practical step-by-step procedure covering cyst selection, hydration, disinfection using sodium hypochlorite (NaOCl), washing and deactivation of residual chlorine, and immediate application of treated cysts. It also highlights key safety precautions, required materials, and recommended operational conditions to ensure effective disinfection while maintaining cyst quality and minimizing risks to operators and the environment. The guidance aims to support improved hatchery biosecurity and more reliable Artemia production. Presented in a highly visual format, the guide transforms the detailed information contained in the FAO Artemia Manual into a practical tool that supports the safe and effective production of high-quality Artemia for aquaculture hatcheries.